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The Matrigel was allowed to polymerize in the incubator at 37 C and 5% CO 2 for 20 min

Step 3 Reconstitution Insert the needle into the BPC-157 + TB-500 vial Inject the water slowly along the vial wall (avoid spraying directly on the powder) Remove the syringe and swirl gently until dissolved ( never shake) Let the vial rest for 35 minutes until the solution clears