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The cell suspension was transferred into a 0.5-mL EP tube, centrifuged at 2000 g for 8 min, and the supernatant was carefully removed

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Finally, glyoxylate is also a twocarbon alphaketo acid that reacts with cyanide to form a cyanohydrin and, more importantly, it can strip cyanide off pyruvate, thereby freeing a central molecule for ATP production [69]