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Proteins extracted from cell lysates using lysis buffer were quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, USA), following the manufacturers instructions

Following protein separation by SDS-PAGE and electrophoretic transfer onto a PVDF membrane, the membrane was incubated overnight at 4 C with primary antibodies specific to MAP2 (Proteintech

2022;5(5): CD006981

The drug also has adverse side effects such as diarrhea and vomiting, "but because these medications are titrated, meaning that the dose is increased gradually, we find a rate at which we can cease that side effect," according to Aronne

10.3390/jcm9010257 J