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The mean distance travelled in the open field was analysed using multiple t -tests, and two-way ANOVA repeated measures were used to analyse habituation to the arena in the open field

Dietary serine supplementation in mice C57BL/6 or Balb/c female mice at 8 weeks of age were purchased from Jiangsu GemPharmatech Co., Ltd (Nanjing, China)

Start low, go slow Beginner approach: Start with single peptide (BPC-157 recommended) Use minimum effective dose Run for 4-8 weeks before adding anything Assess tolerance and results Dose escalation: Begin at lowest recommended dose Increase by 25% every 2-3 weeks if needed Stop increasing when seeing good results More isn't always better Adding peptides: Add one new peptide at a time Wait 2-4 weeks between additions This identifies which peptide causes any issues Proper injection technique Critical for safety: Always use sterile technique Clean injection sites with alcohol Rotate injection sites (prevent tissue damage) Use appropriate needle size (insulin syringes for subcutaneous) Never share needles Infection prevention: New sterile needle every injection Wash hands thoroughly Wipe vial top before drawing Don't touch needle to anything See our complete peptide injections guide for detailed technique

doi: 10.1038/s41467-021-26297-6

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