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Together, these results indicate that PS-MPs affect neurological function via the lungbrain axis, representing an important mechanism underlying PS-MPs-induced cognitive impairment ( Microplastics can cross the bloodbrain barrier, impairing the functions of neuroinflammatory secretion, transporter proteins, and receptor markers, thereby causing neurological damage and subsequently triggering various brain disorders ( The accumulation of polystyrene nanoplastics leads to increased levels of amyloid- (A) and phosphorylated Tau protein (P-Tau) in the cerebral cortex and hippocampus, which are metabolic waste products associated with neurobehavioral disorders and neurodegenerative diseases such as Alzheimers disease

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doi: 10.1158/1541-7786.MCR-16-0480

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Although microbes cannot survive in RGE, it is reasonable to filter the preparations through the 0.22 m filters to remove any potential live microbes before injection