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The URA3 , GFP-tADH1 and HS fragments for GSH1 were ligated between the Sac I and Sal I sites of pRS315 (ATCC) via Gibson assembly using a HiFi DNA assembly cloning kit (New England Biolabs, NEB) according to the manufacturers instructions

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[DOI] [PMC free article] [PubMed] [Google Scholar] 139.Jordan A.J., Manor B., Iloputaife I., Habtemariam D.A., Bean J.F., Sorond F.A., Lipsitz L.A

Modulatory effects of silibinin in various cell signaling pathways against liver disorders and cancer - a comprehensive review