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Studies of protein kinases have shown that mitogen-activated protein kinases (MAPKs) are activated by H2O2 in both animals and plants, which could lead to the modulation of gene expression (Kamata and Hirata, 1999

Deters A, Zippel J, Hellenbrand N, Pappai D, Possemeyer C, Hensel A

If you only had trace amounts after 24 hrs you may have same problem as me, i.e

EAE reflects the autoimmune origin of MS and is probably the most frequently used model that already has been proven as extremely useful to develop/establish/test some of the currently available immunomodulating MS therapeutics (Gold et al., 2000, 2006