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High intakes of BCAAs, including isoleucine, leucine and valine, are independently associated with an increased risk of the progression of coronary artery calcification (72)

& Frye, M

Briefly, rat tail tendon collagen, distilled water, and 4 concentrated DMEM were combined so that the final mixture had 0.75 mg/ml collagen, with a physiological ionic strength of 1 DMEM and a pH of 7.4