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While acetyl-CoA is primarily derived from glucose, OAA is predominantly supplied by glutamine, making glutaminolysis essential for sustaining mitochondrial metabolism, supporting the continuous generation of biosynthetic precursors, and fueling rapid tumor growth ( Figure 3 )

Moreover, the metamorphic climax was the most sensitive stage from a physiological point of view, as there was a decrease in antioxidant defenses and an increase in lipid peroxidation levels