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Schulze RJ, Sathyanarayan A, Mashek DG

10.1111/exd.13548 5 Carvajal-YepesM.HimmelsbachK.SchaedlerS.PloenD.KrauseJ.LudwigL.et al (2011)

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Methods : 6 osteoporotic (OP) and 6 osteoarthritic patients (OA) were enrolled, who underwent surgery for fragility fracture and coxarthrosis, respectively

sgRNA constructs and gene expressing codon optimized (OPT) plasmid constructs sgRNA control and constructs against CBS and L2HGDH were designed targeting GTATTACTGATATTGGTGGG (sgControl) (#230080, Addgene), GGAGCAGACAACCTACGAGG (sg CBS -1) (#230081, Addgene), TGGCAGTGCTGGCAGCACGG (sg CBS -2) (#230082, Addgene), GATATAGTCATCGTTGGTGG (sg L2HGDH -1) (#230083, Addgene), and CACCAGACTGGACATAACAG (sg L2HGDH -2) (#230084, Addgene) and constructed in lentiCRISPR v2-Blast (#83480, Addgene) for Blasticidin selection at a final concentration of 5 g/ml