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Inflammasome and Mitochondrial Status Assessment by Imaging Flow Cytometry Inflammasome complex assembly was evaluated by detection of ASC speck formation by imaging flow cytometry, as previously described ( Flow Cytometry PBMCs were stained with the following list of fluorescently labeled antibodies against cell surface markers subsequently detected by flow cytometry: anti-CD14 BV605 (clone: M5E2), anti-CD16 PE-Cy7/BV711 (clone: 3G8), and anti-CD3 PE (clone: HIT3a) from BioLegend

115 Moreover, CR has been shown to modify the cancer stem cell (CSC) phenotype, reducing their carcinogenic and metastatic potential

Introduction Irons role as the protagonist in ferroptosis centres on its ability to cycle between ferrous and ferric states, enabling electron transfer and redox coupling with reactive oxygen species (ROS) generation such as hydrogen peroxide [1]