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Cells were vortexed for 20 min with intervals and centrifuged for 30 min at 4 C

The increased CoA levels observed in NINJ1 knockdown may result from increased xCT functionality and cystine import, as well as increased PANK1 expression that may increase the de novo synthesis of CoA

This tumour experiment was conducted to test for the non-inferiority of NMN in combination with chemotherapy, versus chemotherapy alone, and to ensure a robust finding was repeated in two independent labs, with data from these separated into Fig