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DNA amplicons were purified using the Quick DNA MiniPrep Plus kit (Zymo Research) and their yield and quality were evaluated using a Nanodrop 1000 spectrophotometer (Thermo Fisher)

It is involved in several processes, such as helping with the detoxification process, supporting the immune system and cardiovascular health, etc

Preclinical studies suggest that ALCAR may increase neuroplasticity (the ability of neurons to create new connections) and neurogenesis (the growth of new brain cells) in the hippocampus (where emotional memories are stored) and prefrontal cortex (higher level thinking and modulation of emotional reactions) (Wang and Pae 2017)

doi:10.7150/ijbs.96476 This issue Cite Review Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China

Clinical research shows that to activate the mTORC1 pathway, a specific "leucine threshold" must be met in a single sitting