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Cellular membranes were pelleted by ultracentrifugation at 100000 g for 1 h, washed with PBS 1X + Triton-X-100 0.1%, resolved by 11% SDS-PAGE, and transferred onto a PVDF membrane

Neuron 20 , 103114 (1998)

MS data acquisition & analysis MS data were acquired by using an Orbitrap Ascend Tribrid MS (Thermo Scientific, v4.0.4091), Vanquish Neo Ultra-High Performance Liquid Chromatography system (Thermo Scientific, v2.1.1.51769), and Aurora Ultimate XT 25 cm 75 ID 1.7 um C18 analytical chromatography columns (IonOpticks, AUR4-25075C18-XT) operated at 55 C

Noticing these signs can help with early detection and proper treatment

So, it is best to limit alcohol consumption if you have MTHFR mutations