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Furthermore, quantitative real-time PCR (qPCR) analysis of melanoma oncogenic genes (CD271, NES, AXL, LOXL2, MITF, MELANA, SOX10) and the melanoma chemotactic gene, CCL2, showed significant downregulation and a change in downstream signalling of these genes by glutamine supplementation in both PDX tumour models (Fig

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For CTG, Calu-3, Huh7.5, and A549 cells were treated with the same concentrations as listed above, and DMSO controls and positive control with 50% DMSO treated cells were added

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