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Selection of GalNAc-conjugated siRNAs with limited off-target-driven rat hepatotoxicity

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doi: 10.1371/journal.pone.0152478 104 KumarA

The supernatant was centrifuged again at 10,000 rcf for 5 min at 4 C

For Nissl staining, the tissues were treated with Cresyl Violet acetate for 15 min at 2226C, rinsed in PBS, dehydrated, transparentized, and mounted prior to microscopic analysis