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Neuroendocrine Impairments of Polycystic Ovary Syndrome Endocrinology

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Although we did not observe any clinical or laboratory evidence of serious hematologic or hepatic toxicity in this study, excessive weight gain was clearly more prevalent in the active treatment group

Intracellular Ca 2+ imaging Cells were transfected using Nucleofector as described before using the plasmids pCMV_R-GECO1 (Addgene #32444, measures intra-cytosolic Ca 2+ , red fluorescence) and pCMV_CEPIA2mt (Addgene #58218, measures intra-mitochondrial Ca 2+ , green fluorescence)