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A suggested strategy is to use 2D cultures for rapid initial screening to identify relevant effects, and 3D models can be helpful for further studies of efficacy or cytotoxicity before using animal models [166]

This stiffness can lead to shortness of breath and increased risk of lung infections

After 2 15 min washes, Alexa 488-conjugated donkey anti-rabbit secondary antibody (Jackson Immunoresearch Europe Ltd., Cambridgeshire, United Kingdom

A mutation in CLCNKB results in hypokalemia as seen in autosomal recessive Bartter syndrome type 3 (BS3) 5355

Macrophages and white blood cells flood the damaged area, and the resulting tissue fluid exudation leads to erythema with a radius of 3 to 5 cm on the skin surface