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Santa Cruz Biotechnology, Inc.) was mixed with 0.75 L SUPERaseIn RNase Inhibitor (Thermo Fisher Scientific) and 1.5 L RNase inhibitor (NIPPON GENE), followed by incubation at 37 C for 4 h

RNAi knockdown of suco-1 or elt-3 suppress SKN-1 activation in amdh-1 mutants, phenocopying the EMS mutants and suggesting a causal relationship between these regulators and SKN-1 (fig2c,d)

J Physiol 443:123136

Severe cases progressing to liver failure may exhibit progressive jaundice, petechiae, ecchymosis, ascites, peritoneal infections, and hepatic encephalopathy