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Dosing is once daily, subcutaneously, with the timing chosen to align with natural lipolytic windows, typically before bed (to coincide with overnight fasting and growth hormone pulses) or first thing in the morning before training and food

Pellets were resuspended in 3 mL of assay buffer (250 mM KCl, 2 mM MgCl 2 , 40 mM HEPES-KOH pH 7.9, 5 mM -mercaptoethanol, 10% (v/v) glycerol, 10 mM imidazole), and 1.25 mL of this resuspension was then used in the pull-down