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From this list, three top candidates were selected for evaluation in mice using the ex vivo mycobacterial growth inhibition assay (MGIA), with one antigen, metE, emerging as a promising candidate due to its strong immunogenic profile

Notably, NOD2 gene mutations are closely associated with structural alterations in the gut microbiota, further demonstrating that IBD development depends on the interaction between the genetic background and microbial communities (100)