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J Biol Chem 296:100187

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The collapsed bam files were processed to bed, bedgraph and bigwig files using samtools 67 , bedtools 68 , and bedGraphToBigWig ( The complete PRO-seq datasets with raw (fastq) and processed (bigWig) files are available via Gene Expression Omnibus database ( Quantification of engaged Pol II at genes and enhancers In PRO-seq, a single biotinylated nucleotide is incorporated into the 3-end of a growing RNA chain, enabling isolation of nascent transcripts from the total pool of RNA, and mapping the active sites of transcription at nucleotide-resolution genome-wide 32,35